AUY922 treatment reduces alloreactive T-cell responses in vivo. Lethally irradiated B6/SJL recipient mice received C3H.SW TCD-BM transplants, together with CD8⁺ T cells that had been prestained with CFSE, followed by AUY922 or phosphate-buffered saline (Con) treatment. Cells were recovered from the recipient mice on day 7 (A-F) and day 14 (A) after transplantation. (A) Graphs show the absolute number of recovered donor CD8⁺ T cells in the spleen and liver. (B) The fraction of apoptotic (Annexin V⁺) donor T cells. (C) The fraction of CFSE^low and CFSE^high cells in donor T cells. (D-E) The expressions of IFN-γ and TNF-α in donor T cells were examined. (F) Levels of serum IFN-γ and TNF-α were measured by enzyme-linked immunosorbent assay. Error bars indicate mean ± standard deviation. Data are representative of 2 to 3 independent experiments. *P < .05; **P < .01; ***P < .001.