Extended treatment of CML cells with IM does not normalize MHC-II or CIITA expression. Primary CD34⁺ CML were treated with 5 µM IM for 7 days. Average surface MHC-II expression levels were determined by flow cytometry, normalized to CML UT (d0) sample for (A) CD34⁺CD38⁺ CML cells and (B) CD34⁺CD38⁻ CML LSCs. (C) The average expression of MHC-II encoding genes HLA-DR, HLA-DP, and HLA-DQ was determined in CD34⁺ CML cells by quantitative reverse transcription polymerase chain reaction (n = 7, calibrated to UT (d0) CML sample). (D) The average gene expression of CIITA was determined in CD34⁺ CML cells by quantitative reverse transcription polymerase chain reaction. Statistical significance was calculated between d0 UT CML sample and all other samples and, if significant, is indicated by asterisks above the bars. Additional comparisons between samples are indicated by lines. NS, not significant.