Figure 2.
Inhibition of c-Kit and effects on erythropoiesis by quizartinib and gilteritinib. (A) TF-1 cells were treated with increasing concentrations of either quizartinib or gilteritinib in RPMI/10% FBS for 1 hour. Immunoprecipitation and immunoblotting were performed to detect the phosphorylation status of c-Kit and total c-Kit. (B) Mononuclear cells isolated from normal donor bone marrow were plated at 105 cells/mL in MethoCult. Increasing concentrations of quizartinib or gilteritinib were added. Counts for colony-forming unit-granulocyte, monocyte (CFU-GM) and burst-forming unit-erythroid (BFU-E) colonies were done after 10 to 12 days of incubation (n = 3). P-c-Kit, phosphorylated c-Kit.
