Effects of p19INK4don human terminal erythroid differentiation. (A) Representative images of flow cytometry analysis of GPA expression in erythroblasts infected with Lucif shRNA or p19INK4d shRNA on day 7; red numbers show statistical analysis of the GPA-positive rate from 3 independent experiments. (B) Representative images of flow cytometry analysis of band 3 and α4-integrin expression on D 7 and D 13 of GPA-positive erythroblasts infected with Lucif shRNA or p19INK4d shRNA. (C) Erythroid cell growth curves determined by manual cell counting of erythroblasts infected with Lucif shRNA or p19INK4d shRNA. (D) The cell-cycle distribution results of BrdU assay from erythroblasts infected with Lucif shRNA or p19INK4d shRNA. (E) Representative images of flow cytometry analysis of apoptosis by annexin V/7AAD staining in erythroblasts infected with Lucif shRNA or p19INK4d shRNA (top). The red numbers indicate percentage. Quantitative analysis from 3 independent experiments is shown (bottom). Statistical analysis of the data from 3 independent experiments and bar plot represents mean ± SD of triplicate samples. *P < .05.
