Klotho administration attenuates IS-induced thrombosis and atherosclerosis in apoE^−/−mice. (A) Flow cytometric analysis of the concentration of PMPs in whole blood (n = 6). (B) Flow cytometric analysis of P-selectin expression in platelets in response to 2 μg/mL collagen (n = 6). (C) Flow cytometric analysis of the percentage of platelet-monocyte aggregates in whole blood in vivo (n = 6). (D) Flow cytometric analysis of platelet-induced CD11b expression in platelet-associated monocytes (n = 6). (E) Flow cytometric analysis of ROS levels in platelets (n = 6). (F) Flow cytometric analysis of the expression of P-p38MAPK in platelets (n = 6). (G) Quantification of the area of platelet adhesion for each group (n = 6). (H) The time for carotid artery occlusion by thrombus for each group (n = 6). (I) Representative images of platelet-monocyte aggregates adhesion on atherosclerotic endothelium as seen by SEM. Black arrows indicate leukocytes adhered on atherosclerotic endothelium. White arrows indicate platelets associated with leukocytes (i). Quantification of the number of platelet-monocyte aggregates for each group (n = 20 fields) (ii). (B-I) Data are mean ± SD. **P < .01 vs vehicle group, ^##P < .01 vs IS group, ANOVA. (J) Representative cross-sections (hematoxylin and eosin staining) of the aortic sinus. Scale bar, 100 μm. (K) Quantification of the atherosclerotic plaque area in aortic root (n = 6). Data are mean ± SD. **P < .01 vs vehicle group, ^##P < .01 vs IS group, ANOVA.