Figure 5.
Figure 5. Xenogeneic suppression of anti-FVIII antibody response in vivo by human ANS8 CAR Tregs. (A) Experimental schema. Female E16×DR1 mice (n = 5 per group) were subcutaneously immunized with FVIII in incomplete Freund’s adjuvant on day 0. Four hours after immunization, the mice were adoptively transferred with GFP+ Tregs expressing ANS8 CAR, 17195 TCR, or control Ob2F3 TCR. The anti-FVIII antibody levels were followed weekly after the immunization (dotted arrows). Mice received one additional challenge with FVIII, together with 10% TNP-SRBC to test antigen-specificity of tolerance on day 56 (solid arrows). (B) Time course of anti-FVIII antibody response. Data indicate mean ± SEM. Multiple Student t tests were performed to determine statistical significance. *P < .05, **P < .01.

Xenogeneic suppression of anti-FVIII antibody response in vivo by human ANS8 CAR Tregs. (A) Experimental schema. Female E16×DR1 mice (n = 5 per group) were subcutaneously immunized with FVIII in incomplete Freund’s adjuvant on day 0. Four hours after immunization, the mice were adoptively transferred with GFP+ Tregs expressing ANS8 CAR, 17195 TCR, or control Ob2F3 TCR. The anti-FVIII antibody levels were followed weekly after the immunization (dotted arrows). Mice received one additional challenge with FVIII, together with 10% TNP-SRBC to test antigen-specificity of tolerance on day 56 (solid arrows). (B) Time course of anti-FVIII antibody response. Data indicate mean ± SEM. Multiple Student t tests were performed to determine statistical significance. *P < .05, **P < .01.

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