Figure 5.
Figure 5. Trogocytosis by PMN is not accompanied by CLL B-cell death. Purified PMNs were cocultured with CLL B cells at a 1:3 E:T ratio and 10 µg/mL of anti-CD20 antibodies RTX-WT, RTX-GE, CET control antibody, or no mAb. At 3 (3H) and 20 hours (20H) (A-B) or at day 7 (C), anti-CD19 allophycocyanin, 7AAD, and calibration beads were added to measure the absolute number of total CLL B cells at different times (in panels A and C) or the percentage of dead 7AAD+ CD19+ CLL B cells (in panel B), respectively. The results are the means and standard deviations of 4 to 6 independent experiments.

Trogocytosis by PMN is not accompanied by CLL B-cell death. Purified PMNs were cocultured with CLL B cells at a 1:3 E:T ratio and 10 µg/mL of anti-CD20 antibodies RTX-WT, RTX-GE, CET control antibody, or no mAb. At 3 (3H) and 20 hours (20H) (A-B) or at day 7 (C), anti-CD19 allophycocyanin, 7AAD, and calibration beads were added to measure the absolute number of total CLL B cells at different times (in panels A and C) or the percentage of dead 7AAD+ CD19+ CLL B cells (in panel B), respectively. The results are the means and standard deviations of 4 to 6 independent experiments.

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