Following FeCl₃injury, control and RBC^HIGHmice have similar thrombus morphology and similar levels of circulating TATc. (A-B) Thrombi from a subset of mice subjected to FeCl₃-induced carotid artery thrombosis were excised, fixed in 10% formalin, transferred to 70% ethanol, and analyzed. (A) Hematoxylin and eosin (H&E) staining (upper panels) and immunohistochemistry for fibrin (brown staining; lower panels). Scale bars indicate 500 µm. (B) Thrombus size was determined by measuring pixel area of the thrombus within the vessel. (C) TATc were measured by enzyme-linked immunosorbent assay in plasmas from control (uninfused) and RBC^HIGH mice following FeCl₃-induced artery occlusion. (D) Relationship between hematocrit and TATc levels measured after FeCl₃-induced artery occlusion in control (open circles) and RBC^HIGH (closed circles) mice. In panels B-D, each dot is a separate mouse. In panels B-C, horizontal lines indicate medians.