Comparative analysis of the platelet (PLT) and megakaryocyte transcriptomes. (A) Number of unique transcripts identified by RNA-Seq in megakaryocytes (Nürnberg et al, 2012)61 and platelets (this study). (B) Transcript abundance in megakaryocytes and platelets based on RNA-Seq data. Yellow inset indicates transcripts expressed above the determined threshold level (0.3 or 0.1 RPKM). Marginal histograms show the expression of discordant transcripts in megakaryocytes and platelets (gray and red) or shared transcripts (blue). (C) Histograms of platelet enrichment scores (platelet RPKM/MK RPKM; blue) or RNA-Seq replicates (platelet replicate 1 RPKM/platelet replicate 2 RPKM; red). (D) Identical analysis as in panel C performed for in vitro–derived PLPs and corresponding Meg01 samples.
