Expression of podoplanin in the IVC vessel wall increases with thrombosis. (A-C) Thrombi and the IVC were taken separately for western blotting. (A) Western blot of 5 thrombi vs 1 IVC after 48 hours of stenosis. Note complete absence of podoplanin in 4 of 5 thrombi and abundant podoplanin signal in the IVC. (B) Intact IVCs (lanes 1-3), IVCs after stenosis with thrombi (St+T, lanes 4-6), IVC after stenosis without thrombi (St N/T, lanes 7-9). Loading is confirmed by blotting for tubulin. (C) Densitometry of podoplanin bands normalized to tubulin; data in bar graph represent mean ± SD; n = 6 for each group. Ctrl, control.