Impact of IL-4 on stromal cells committed to lymphoid stroma. (A-B) ADSCs were primed or not for 3 days with TNF/LT before treatment with TNF/LT, IL-4, or nothing (UNT) for 3 additional days and quantification of CXCL12, TGM2, and VCAM1 by RT-qPCR. The arbitrary value of 1 was assigned to ADSC maintained without any cytokine during all of the 6-day experiments. In addition, CXCL12 concentration was assessed by ELISA. Results represent the mean ± SD from 6 to 7 experiments. (C) ADSCs were primed or not for 24 hours with TNF/LT, starved for 2 hours, and then treated with IL-4 for 5 minutes. STAT6 activation was first evaluated by flow cytometry (left) and quantified as the RMFI of pSTAT6 staining in IL-4–treated vs IL-4–untreated cells (n = 6). In addition, STAT6, pSTAT6, and β-actin expression was determined by western blot, and 1 representative experiment of 2 is shown. ns, not significant. *P < .05.