KLHDC8B knockdown mimics TET3 knockdown-induced defects in enucleation, and nuclear abnormalities and ectopic expression of KLHDC8B partially rescued bi/multinucleation defect. (A) Representative profiles of enucleation as assessed by Syto-16 staining on day 17 of culture. Enucleation percentage was calculated as a Syto-16–negative population in the total population. (B) Quantitative analysis of enucleation on indicated days from 3 independent experiments. (C) Representative cytospin images of erythroblasts on day 17 stained with May-Grünwald Giemsa. Scale bar = 10 μm. (D) Quantification of bi/multinucleated erythroblasts in KLHDC8B shRNA-transduced cells. (E) Western blot analysis of KLHDC8B expression. (F) Quantification of KLHDC8B ectopic expression, with GAPDH as control. (G) Quantification of bi/multinucleated erythroblasts following the ectopic expression of KLHDC8B, with empty vector pGFP as control. *P < .05; **P < .01; ***P < .001. D, days; FSC, forward scatter; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; pGFP, plasmid encodes green fluorescent protein; pKLHDC8B, plasmid encodes KLHDC8B and green fluorescent protein.