Comparison of GPI presence in different blood cell lineages from PNH patients. (A) Representative FLAER staining analysis on blood cell populations from patient 6, showing FLAER⁻ (GPI^neg) and FLAER⁺ (GPI^pos) neutrophils (CD15⁺), NK cells (CD3⁻CD19⁻CD14⁻CD56⁺), and T cells (CD3⁺). Separate analyses for CD56^bright and CD56^dim NK cells are shown. (B) Bar graph depicts the fraction of GPI^neg granulocytes for each patient. Patient samples are ordered by contribution of GPI^neg cells to the neutrophil compartment starting with largest clone size. (C-E) Bar graphs depict the fraction of GPI^neg cells in other lineages, (C) CD56^bright NK cells, (D) CD56^dim NK cells, and (E) CD3⁺ T cells. Patient number and CMV status (IgG⁺ or IgG⁻) are indicated. (F) Longitudinal analysis of the fraction of GPI^neg cells in each lineage over time in patient 15. The x-axis indicates the time postdiagnosis and the y-axis the fraction of GPI^neg cells. FSC, forward scatter.