American Society of Hematology

Figure 5.

$Figure 5. CD99 is required on endothelial cells but not on neutrophils for extravasation from cremaster venules. WT- and CD99−/−-recipient mice were lethally irradiated and transplanted with donor bone marrow as follows: WT donors into WT recipients (red bars), CD99−/− donors into WT recipients (blue bars), and WT donors into CD99−/− recipients (light green bars). Six weeks later, mice were analyzed by intravital microscopy of the cremaster after 2 hours of TNF-α stimulation for (A) rolling flux fraction, (B) rolling velocity, (C) numbers of adherent leukocytes, and (D) extravasated leukocytes (supplemental Table 2 shows hemodynamic parameters). Results are displayed as mean ± standard error of the mean for 5 mice per group, with a total of 35, 33, and 34 vessel segments per group, respectively. ***P < .001 and n.s. as per 1-way analysis of variance. PMN, polymorphonuclear neutrophil.$

CD99 is required on endothelial cells but not on neutrophils for extravasation from cremaster venules. WT- and CD99^−/−-recipient mice were lethally irradiated and transplanted with donor bone marrow as follows: WT donors into WT recipients (red bars), CD99^−/− donors into WT recipients (blue bars), and WT donors into CD99^−/− recipients (light green bars). Six weeks later, mice were analyzed by intravital microscopy of the cremaster after 2 hours of TNF-α stimulation for (A) rolling flux fraction, (B) rolling velocity, (C) numbers of adherent leukocytes, and (D) extravasated leukocytes (supplemental Table 2 shows hemodynamic parameters). Results are displayed as mean ± standard error of the mean for 5 mice per group, with a total of 35, 33, and 34 vessel segments per group, respectively. ***P < .001 and n.s. as per 1-way analysis of variance. PMN, polymorphonuclear neutrophil.

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