Kinetics of increased surface marker expression on T cells and CD8⁺ DCs after TLI in wild-type and Batf3^−/−mice. (A) Representative FACS patterns of PD-1 on gated CD4⁺CD25⁻ conventional T cells (Tcon), or PD-1 on gated CD8⁺ T cells, or PD-1 on gated CD4⁺CD25⁺ Tregs from untreated (n = 8) or TLI-treated wild-type mice (n = 11). (B) Similar stainings are shown in untreated (n = 5) or TLI-treated Batf3^−/− mice (n = 10). (C-E) Percentages of PD-1⁺ cells among T-cell subsets from untreated WT or Batf3^−/− mice or from TLI-treated WT or Batf3^−/− mice. (F) Percentages of gated splenic CD8⁺ DCs that expressed Tim-4, DEC205, Treml4, or SIRPα before, during, or after 10 daily doses of TLI conditioning. (G) Percentages of gated NKT, Tregs, conventional CD4⁺ and conventional CD8⁺ T cells that expressed PD-1 or NKG2D at the different time points shown in panel F.