TLI conditioning that favors the accumulation of CD8⁺ DCs is dependent on NKT cells. (A) Representative 2-color FACS patterns of CD8α vs CD11b on gated CD11c⁺ spleen cells from untreated (UNT) wild-type (n = 6) or Jα18^−/− (n = 5) or Batf3^−/− (n = 5) or from TLI-conditioned wild-type (n = 11), Batf3^−/− (n = 7), or Jα18^−/− (n = 8) BALB/c mice, at day 5 after completion of TLI. Percentages of DC subsets in each box are shown. (B) Mean (± standard error of the mean [SEM]) percentages of: CD11c⁺CD11b⁻CD8α⁺ cells (C), CD11c⁺CD11b⁺CD8α⁻ cells (D), CD11c⁺CD11b⁻CD8α⁻ cells (E). Representative 2-color FACS pattern of 7AAD vs Annexin V staining of spleen cells immediately after completion of 10 doses of TLI in a BALB/c mouse. SSC, side scatter.