Figure 5.
Figure 5. Competitive transplantation of CD41−/+/Hi HSCs after G-CSF treatment. (A) Experimental schematic. UBC-GFP mice were treated with G-CSF for 4 days. Three hours after the final treatment, BM was harvested and the CD41−/+/Hi cells from within the HSC compartment were sorted and competitively transplanted. (B) Five-lineage repopulation of mice transplanted with 20 CD41−/+/Hi cells. Horizontal line represents threshold for repopulation. (C) Chart summarizing data from B. (D) Five-lineage repopulation of mice transplanted with 100 CD41+/Hi cells. (E) Chart summarizing data from D. (F) Analysis of the BM HSPC compartment 16 weeks after transplantation with 20 CD41−/+ cells. Only mice that demonstrated PB reconstitution were analyzed. (G) Analysis of the BM HSPC compartment 16 weeks after transplantation with 100 CD41+/Hi cells. All mice were analyzed, regardless of PB reconstitution.

Competitive transplantation of CD41−/+/HiHSCs after G-CSF treatment. (A) Experimental schematic. UBC-GFP mice were treated with G-CSF for 4 days. Three hours after the final treatment, BM was harvested and the CD41−/+/Hi cells from within the HSC compartment were sorted and competitively transplanted. (B) Five-lineage repopulation of mice transplanted with 20 CD41−/+/Hi cells. Horizontal line represents threshold for repopulation. (C) Chart summarizing data from B. (D) Five-lineage repopulation of mice transplanted with 100 CD41+/Hi cells. (E) Chart summarizing data from D. (F) Analysis of the BM HSPC compartment 16 weeks after transplantation with 20 CD41−/+ cells. Only mice that demonstrated PB reconstitution were analyzed. (G) Analysis of the BM HSPC compartment 16 weeks after transplantation with 100 CD41+/Hi cells. All mice were analyzed, regardless of PB reconstitution.

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