Cytotoxic granule constituent and T-bet expression pattern resembling adaptive NK cells in patients with GATA2 mutation. PBMCs, healthy donors, and individuals with heterozygous GATA2 mutations were examined for expression of cytotoxic granule constituents perforin, granzyme A (GzmA), and granzyme B (GzmB) as well as the transcription factors T-bet and Eomes by intracellular flow cytometry. Expression is shown as median fluorescence intensity relative to (R-MFI) CD3⁺CD8⁺CD57⁺ T cells in the same sample. (A) The graph depicts expression of perforin, granzyme A and B in conventional PLZF⁺ (open circles) and adaptive PLZF⁻ (filled circles) CD3⁻CD56^dim NK cells from healthy adult CMV-seropositive donors (n = 16) with sizeable pools of adaptive NK cells. (B) The graphs depict perforin, granzyme A and B expression in CD3⁻CD56^dim NK cells from healthy CMV-seronegative (n = 6) and -seropositive donors (n = 6), as well as patients (n = 8) or asymptomatic carriers (n = 2) with GATA2 mutation. (C) The graph depicts expression of T-bet and Eomes in conventional PLZF⁺ (open circles) and adaptive PLZF⁻ (filled circles) CD3⁻CD56^dim NK cells from healthy adult CMV-seropositive donors with sizeable pools of adaptive NK cells (n = 16). (D) The graphs depict T-bet and Eomes expression in CD3⁻CD56^dim NK cells from healthy CMV-seronegative (n = 6) and -seropositive donors (n = 6), as well as patients (n = 6) or asymptomatic carriers (n = 2) with GATA2 mutation. Mean is indicated, with error bars representing SD. P values were determined using the Student (A,C) paired and (B,D) unpaired t test. **P < .01 and ***P < .001.