Effect of CRISPR gene knockout on apilimod-induced lysosomal expansion. (A) LysoTracker staining in WSU-DLCL2 CRISPR knockout pools after 48-hour treatment with 200 nM apilimod (blue) compared with DMSO-treated control (red). Three independent sgRNA pools for each gene listed are displayed. (B) Corresponding images of vacuole formation in the cells are displayed (original magnification ×40). Arrows highlight vacuoles. Images sharpened evenly in order to highlight vacuoles present in floating lymphoma cells.