Kinetics of monoadducts and ICLs repair and chromatin condensation in BMPCs. (A) The kinetics of monoadducts repair 0 to 2 hours (A) and 2 to 48 hours (B) after treatment with melphalan alone. R, responders; NR, nonresponders; NO INH, no inhibitor. The kinetics of monoadducts repair 2 to 48 hours after treatment of BMPCs from responders (C) or nonresponders (D) with melphalan alone or in combination with an inhibitor. (E) Total amounts of monoadducts, expressed as AUC, after treatment with melphalan in the presence or not of an inhibitor. Representative autoradiograms showing chromatin condensation of 3 untreated responders (R1, R2, R3) and 3 nonresponders (NR1, NR2, NR3) in the FN3 (F-G) and FN6 (H-I) fragments of the N-ras gene locus. Symbols M, D, and T represent the positions of nucleosome monomer, dimer, and trimer, respectively. (J) Kinetics of ICL formation and repair after treatment with melphalan alone. Formation and repair of ICLs 2 to 48 hours after treatment of BMPCs from responders (K) or nonresponders (L) with melphalan in combination or not with an inhibitor. (M) Total amounts of ICLs, expressed as AUC, after treatment with melphalan in the presence or not of an inhibitor. The experiments shown were based on a minimum of 3 independent repeats and the data reported are the mean ± SD of all the patients analyzed. Bar graph and error bars show mean ± SD.