Kinetics of monoadducts repair in MM cell lines. (A) Representative autoradiograms for the Southern blot analysis of monoadducts. 0/0, no treatment. A horizontal line has been inserted to indicate a repositioned gel lane. The kinetics of monoadducts repair 0 to 2 hours (B) and 2 to 48 hours (E) after treatment with melphalan alone are shown. NO INH, no inhibitor. The kinetics of monoadducts repair 0 to 2 hours (C-D) and 2 to 48 hours (F-G) after treatment of RPMI 8226 (C,F) and LR5 (D,G) cells with melphalan in the presence or not of an inhibitor are also presented. (H) Total amounts of monoadducts, expressed as AUC, after treatment with melphalan in the presence or not of an inhibitor. (I) The gene locus where the kinetics of DNA repair and chromatin condensation were evaluated. Representative autoradiograms showing chromatin condensation of the untreated RPMI 8226 and LR5 cells in the FN3 (J) and FN6 (K) fragments of the N-ras gene locus. Vertical lines have been inserted to indicate a repositioned gel lane. Symbols M, D, and T represent the positions of nucleosome monomer, dimer, and trimer, respectively. The experiments shown were based on a minimum of 3 independent repeats. Bar graphs and error bars show mean ± standard deviation (SD).