Representative samples showing podoplanin expression and intravascular platelet clusters (antiplatelet surface protein CD61) in brain tumor specimens. (A-B) Representative examples of high podoplanin (+++)-expressing tumors. (C) Representative example of a podoplanin-negative (–) tumor. (D) The invasion zone of a podoplanin-expressing tumor is depicted. (E) A very large CD61⁺ intravascular thrombosis (+++) is shown. (F) Tumor sample with multiple CD61⁺ thrombotic vessels (++). NDP Viewing Software (NDP.view2; Hamamatsu Photonics, Japan) was used for microscopy photographs. Full scale bars represent 500 μm (A), 250 μm (B-C,E-F), and 2.5 μm (D).