Non–cell-intrinsic effects of miR-125b in AF9+Ri125b leukemia. (A) Schematics of the transwell coculture experiment, in which AF9-only leukemia cells were cocultured with AF9+Ri125b AML leukemia cells in the presence or absence of Dox. (B) Cell numbers and (C-D) apoptosis levels were determined for the described experiment in (A) 2 days after plating cells (n = 3; *P < .01; **P < .001). Representative flow cytometry plots are shown in (C). (E) Schematic of cotransplantation of AF9-only AML cells and AF9+Ri125b AML cells. Wild-type recipient mice were treated with or without Dox. All mice were analyzed on day 28 for leukemia cells in right femur bone. (F) Total AF9-only leukemia cells or AF9+Ri125b leukemia cells in the right femur bones were plotted, with each dot representing 1 mouse (n = 5; error bars represent standard deviations; *P < .05; **P < .01).