MMB modulates hepcidin expression and iron metabolism gene expression in ACD in rats. (A-B) Serum iron, hepcidin serum protein, and liver Hamp messenger RNA (mRNA) levels in ACD rats after 3-day MMB (5, 10, or 25 mg/kg) treatment. (C) IL-6 mRNA levels in the spleen of ACD rats after 3-day MMB (5, 10, or 25 mg/kg) treatment. (D) Representative western blots and quantification (n = 4 per group) of FPN1, transferrin receptor 1 (TfR1), and ferritin in the spleens and livers of ACD rats after 3-day MMB (5, 10, or 25 mg/kg) treatment. β-actin levels were used for normalization. Each lane represents one individual animal. Analysis of variance with Dunnett’s correction for multiple comparisons vs ACD was applied (A-C). Unpaired Student t test was applied for comparison in each group (D). Results are shown as means ± standard errors of the mean. *P < .05, **P < .01, ***P < .001.