MMB ameliorates anemia in a rat model of ACD. (A-B) Hb, RBC count, mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), white blood cell count (WBC), and neutrophil count in ACD rats after MMB (25 mg/kg) treatment for 21 days (day 0; corresponds to 14 days after PG-APS injection). (C-E) Representative FACS dot plots from rat bone marrow after 3 weeks of MMB (5, 10, or 25 mg/kg) treatment. FACS analysis was performed with freshly isolated bone marrow cells 6 hours after the last administration of MMB. The total numbers (D) as well as percentages (E) of the different erythropoietic populations per femur are shown as means ± standard errors of the mean (more details provided in supplemental Figure 2). (F) Serum iron levels after MMB (25 mg/kg) treatment. Unpaired Student t test was applied for comparison at each time point (A-B,F) and analysis of variance with Dunnett’s correction for multiple comparisons vs ACD (D-E). *P < .05, **P < .01, ***P < .001. FSC, forward scatter.