DKO mice maintain vascular integrity during inflammation of the skin and lung. (A-B) WT, platelet-depleted WT, WT treated with JON/A F(ab)2 to block integrin GPIIb/IIIa, and DKO mice were subjected to the rpA reaction to induce local skin inflammation. (A) Representative images. Inflammatory spots are highlighted. (B) Quantification of the Hb content in tissue punch biopsies from inflammatory spots. (C) Quantification of the Hb content in tissue punch biopsies from platelet-depleted hIL-4Rα/GPIbα–Tg mice transfused with WT or DKO platelets or untransfused mice subjected to the rpA reaction. (D-E) WT, platelet-depleted WT, and DKO mice were subjected to LPS-induced lung inflammation. (D) Representative images of BAL 4 hours after LPS application. (E) Quantification of the Hb content in BAL liquid. Results are presented as mean ± SD. n = 4 mice per group, representative of 3 independent experiments. ***P < .001. Plt depl, platelet depleted; SD, standard deviation.
