MOF histone acetyltransferase activity is required for adult hematopoietic cell survival. (A) Schematic of full-length MOF, CoA-binding domain deleted MOF, or G327E mutant MOF. (B) Schematic for in vitro rescue experiment. BM LSKs derived from 4 Mof^f/f or 4 WT mice were pooled by genotype and retrovirally transduced with full-length Mof (miCD2-Mof), a Mof mutant (miCD2-CoAdel or miCD2-G327E), or empty vector (miCD2), and hCD2⁺ cells were sorted after 48 hours. These cells were then infected with dTomato-Cre, sorted another 48 hours (hr) later and used for CFU assays. (C) Western blot showing global H4K16ac, H4, and actin in Mof^f/f and WT miCD2, miCD2-Mof, miCD2-Mof-CoAdel and miCD2-Mof-G327E cells at 48 hours after Cre transduction. (D) Day 10 of CFU assay. In all, 7000 cells were plated per dish. Representative petri dishes are shown. (E) Day 10 of CFU assay. Bar graph indicates mean number of colonies per dish after 10 days (left) or mean number of live cells (right) per dish. Data are representative of 2 individual experiments. (F) Mean percentage of the various colony types relative to all colonies counted per dish per genotype. (G) PCR analysis illustrating Mof excision in hCD2⁺, dTomato⁺ LSKs 48 hours after Cre infection and at day 10 of the colony-forming assay. A representative image is shown.