Vav1-cre–induced homozygous Mof loss does not affect fetal hematopoiesis at E14.5. (A) Representative photographs of Vav1-cre;Mof^f/f, Vav1-cre;Mof^f/+, and Mof^f/f embryos at E14.5. (B) Total live FL cell count. (C) PCR analysis illustrating Mof excision in fresh CD45.2⁺ FL cells. A representative image is shown. (D) Western blot showing global H4K16ac, H4, and actin in fresh CD45.2⁺ FL cells. (E) Percentages of mature B cells (B220⁺), myeloid cells (GR1⁺), and T cells (CD3⁺) in live FL cells as measured by FACS. (F) Percentages of cells at various stages of differentiation within the erythroid lineage in live FL cells as measured by FACS. (G) Percentages of MPs and LSKs in live, Lin^– FL cells, and HSCs (CD150⁺/CD48^–) as a percentage of LSKs, all measured by FACS. Error bars represent SD of mean.