Vav1-cre-Mof^f/fP9 hematopoietic bone marrow cells are functionally impaired. (A) Day 10 of CFU assay of fresh, CD45.2⁺Vav1-cre;Mof^f/f, Vav1-cre;Mof^f/+, and Mof^f/f P9 BM cells. Bar graphs indicate mean number of colonies after 10 days (left) and number of live cells (right) per plating. In all, 20 000 cells were plated per dish. Data are representative of 3 individual experiments with multiple donor mice per experiment. (B) Mean percentage of the various colony types relative to all colonies counted per dish per genotype. (C) Schematic for transplant experiment with CD45.2⁺Vav1-cre;Mof^f/f, Vav1-cre;Mof^f/+, and Mof^f/f P9 BM cells. B6.SJL recipient mice were injected with BM cells shortly after lethal irradiation (2 × 5 Gy). The experiment was repeated 3 times with multiple donor mice per genotype per experiment. (D) Survival curve of recipient mice. Mice were all euthanized 16 weeks (wks) posttransplant. Vav1-cre;Mof^f/f, n = 4; Vav1-cre;Mof^f/+, n = 8; Mof^f/f, n = 9. (E) Bar graph illustrating the percentage of donor cells (CD45.2⁺) present in recipient BM comparing the Vav1-cre;Mof^f/f recipient mice (for which proper engraftment failed) to a properly engrafted Vav1-cre;Mof^f/+ recipient mouse. (F) Percent of CD45.2 live cells in PB over the time-course of the transplant experiments. (G) PCR analysis illustrating Mof excision in CD45.2⁺ BM cells at time of euthanasia. A representative image is shown. Error bars represent SD of mean. Significance is shown for comparing Vav1-cre;Mof^f/f and Vav1-cre;Mof^f/+ to Mof^f/f. *P < .05. CFU-GEMM, CFU–granulocyte, erythroid, macrophage, megakaryocyte; CFU-GM, CFU–granulocyte, macrophage; CFU-M, CFU-macrophage; CFU-G, CFU-granulocyte; BFU-E, burst forming unit-erythroid.