American Society of Hematology

Figure 3.

$Figure 3. Reciprocal activation of FXII and PK in the absence of a surface. (A-C) FXII (200 nM) and PK (200 nM) species were incubated at 37°C. At indicated time points, samples were removed into reducing (A,C) or nonreducing (B) sample buffer, size fractionated by SDS-PAGE, and analyzed by western blot using (A,C) polyclonal IgG to FXII (XII) or PK or (B) monoclonal IgGs that preferentially recognize the activated forms of FXIIa (D06) and kallikrein (H03). (A-B) Reciprocal activation of plasma FXII and PK. (C) Activation of PK-WT by recombinant FXII species. For panels A and C, positions of standards for FXII (XII) and the heavy chain (HC) and light chain (LC) of FXIIa; and standards for PK, the heavy chain and light chain of α-kallikrein, and a fragment of the heavy chain of β-kallikrein (β) are indicated at the right of each image. For panel B, positions of standards for αFXIIa, βFXIIa, α-kallikrein (α-kal), and β-kallikrein (β-kal) are indicated on the right.$

Reciprocal activation of FXII and PK in the absence of a surface. (A-C) FXII (200 nM) and PK (200 nM) species were incubated at 37°C. At indicated time points, samples were removed into reducing (A,C) or nonreducing (B) sample buffer, size fractionated by SDS-PAGE, and analyzed by western blot using (A,C) polyclonal IgG to FXII (XII) or PK or (B) monoclonal IgGs that preferentially recognize the activated forms of FXIIa (D06) and kallikrein (H03). (A-B) Reciprocal activation of plasma FXII and PK. (C) Activation of PK-WT by recombinant FXII species. For panels A and C, positions of standards for FXII (XII) and the heavy chain (HC) and light chain (LC) of FXIIa; and standards for PK, the heavy chain and light chain of α-kallikrein, and a fragment of the heavy chain of β-kallikrein (β) are indicated at the right of each image. For panel B, positions of standards for αFXIIa, βFXIIa, α-kallikrein (α-kal), and β-kallikrein (β-kal) are indicated on the right.

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