Figure 4.
Minor contribution of NET-platelet interaction to microvascular platelet adhesion. (A) Representative SD-IVM image of the liver microvasculature of an endotoxemic wild-type (PAD4+/+) mouse showing neutrophils (green, AF488 anti-Gr1), NETs (red, AF555 anti-H2Ax), and platelets (blue, AF647 anti-CD49b). Bar represents 50 μm. (B) Magnified inset showing platelets (blue) aggregating on the surface of an adherent neutrophil (green). (C) Magnified inset showing platelets (blue) adhering to a NET (red). (D) Quantitative analysis of platelet adhesion within the liver sinusoids of wild-type (PAD4+/+) and PAD4−/− mice. Data are represented as mean ± SEM. *P < .05; N = 4-6 mice per group.

Minor contribution of NET-platelet interaction to microvascular platelet adhesion. (A) Representative SD-IVM image of the liver microvasculature of an endotoxemic wild-type (PAD4+/+) mouse showing neutrophils (green, AF488 anti-Gr1), NETs (red, AF555 anti-H2Ax), and platelets (blue, AF647 anti-CD49b). Bar represents 50 μm. (B) Magnified inset showing platelets (blue) aggregating on the surface of an adherent neutrophil (green). (C) Magnified inset showing platelets (blue) adhering to a NET (red). (D) Quantitative analysis of platelet adhesion within the liver sinusoids of wild-type (PAD4+/+) and PAD4−/− mice. Data are represented as mean ± SEM. *P < .05; N = 4-6 mice per group.

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