Novel ITK-FER kinase fusion in PTCL, NOS. (A) ITK-FER rearrangement as detected by MPseq. (B) ITK-FER fusion transcript as detected by RNAseq. (C) Sanger sequencing of the ITK-FER fusion site. (D) Exon-level RNAseq read plot for FER in TCL72 (“Case”) compared with the remaining 10 sequenced cases of PTCL, NOS. RPKM, reads per kilobase per million. (E) Schematic diagrams of protein domains in ITK, FER, and the ITK-FER fusion protein. (F) ITK-FER expression promotes colony formation in HEK-293 cells. PH, pleckstrin homology; TH, Tec homology; SH, Src homology; F-BAR, FER-CIP4 homology–Bin-amphiphysin-Rvs.