Continuous single-cell analysis reveals cell-fate differences between supportive and nonsupportive conditions. (A) Cell-fate quantification of founder HSCs cocultured with different stroma: supportive AFT024 (dark gray bars, n = 7 independent experiments, 290 trees), intermediately supportive 2012 (n = 3 independent experiments, 129 trees), and nonsupportive 2018 (white bars, n = 5 independent experiments, 264 trees). (B) Quantification of dividing HSC rates on different stroma over the first 3 generations. (C) Cell-fate quantification of founder early (n = 5 independent experiments, 274 trees) and late MPPs (n = 4 independent experiments, 211 trees) compared with HSCs cultured on supportive AFT024 stroma. (D) Cumulative time curves representing absolute time required for HSC/MPP division on AFT024 stroma. (E) Quantification of most representative HSC/MPP tree genealogies cultured on AFT024 stroma (up to generation 1).