KRK α₁AT rescues the effect of soluble TM in normal and factor-deficient human plasma. (A-B) Representative thrombin generation curves showing the effect of KRK α₁AT (concentrations indicated) in normal human plasma (NP) in the (A) absence and (B) presence of 1.25 nM soluble TM. Each curve is an average of duplicates. In (B) the no TM control from (A) is shown for reference. (C) Bar graph of total thrombin generation in normal plasma (given as thrombin concentrations) at 0, 1.25, and 10 nM TM, with increasing concentrations of KRK α₁AT (up to 2 μM). Each bar is the average of 3 experiments of duplicates, with standard deviation shown by vertical lines. (D) Bar graph of total thrombin generation in fVIII-deficient (hemophilia A) plasma (given as thrombin concentrations) at 0, 1.25, and 5 nM TM, with increasing concentrations of KRK α₁AT (up to 2 μM). Each bar is the average of 2 experiments of duplicates with standard deviation shown. (E) Bar graph of total thrombin generation in fIX-deficient (hemophilia B) plasma (given as thrombin concentrations) at 0, 1.25, and 5 nM TM, with increasing concentrations of KRK α₁AT (up to 2 μM). Each bar is the average of at least 2 experiments of duplicates, with standard deviation shown.