Significantly decreased erythroferrone in double-heterozygote mice. (A) Erythroferrone mRNA expression in erythroid-enriched bone marrow from WT, TfR1^+/−, th3/+, and double-heterozygote mice (n = 3-6 mice per group). Concurrently assessed serum erythroferrone (B) and hepcidin (C) concentrations in WT, TfR1^+/−, th3/+, and double-heterozygote mice (n = 5-6 mice per group). *P < .05; **P < .01 vs WT mice; †P < .05 vs th3/+ mice. ND, not detected.