Recruitment of HDAC1, HDAC2, and KDM1 to the promoters of BTK-targeting miRs in CLL. (A) Co-immunoprecipitation (IP) of HDAC1, HDAC2, and KDM1 from the nuclei of 3 CLL samples in comparison with IgG. Figure is representative of 3 experiments. (B) Relative recruitment of HDAC1, HDAC2, and KDM1 in comparison with IgG at the miR-147b, miR-210, miR-425, miR-1253, miR-4269, miR-4667-3p, CDKN1a (gene regulated by HDACs used as a positive control), and miR-622 (gene predicted to target BTK but not regulated by HDACs used as negative control) promoters in CLL samples with adverse cytogenetics. Data representative of 3 individual CLL samples analyzed by ChIP-Seq. (C-E) Recruitment of HDAC1, HDAC2, and KDM1 at miR-147b, miR-210, miR-425, miR-1253, miR-4269, miR-4667-3p using primers specific or nonspecific (NS) to the promoters for these miRs. Data represent mean ± SEM of 6 CLL samples.