Overview of driver gene mutations identified by targeted sequencing in 664 AML patients. (A) Histogram showing the frequency of driver gene mutations detected in >1% of patients. Bars are colored according to the functional category assigned to each driver gene. (B) Number of mutated driver genes per patient, according to age category (<40 years, n = 112; 40-59 years, n = 264; ≥60 years, n = 288). (C) Number of mutated driver genes per patient, according to MRC cytogenetic risk category (favorable, n = 65; intermediate, n = 452; adverse, n = 129). (D) Number of mutated driver genes per patient for patients with de novo AML (n = 570) compared with sAML (n = 59) or tAML (n = 35). P values in (B-D) were calculated by the Kruskal-Wallis test, without adjustment for multiple testing.