Figure 4
CD19-CAR–modified TSCM have a transcriptome profile similar to their naturally occurring counterpart. (A) Hierarchical clustering of CD19-CAR–modified standard and TSCM-enriched products and naturally occurring CD8+ T-cell subsets from GSE23321 performed using a 900 gene list from Gattinoni et al.9 Red and blue colors indicate increased and decreased expression of 900 differentially regulated genes described by Gattinoni et al.9 Each column represents a sample and each row, a gene. (B) Principal component analysis (PCA) of the 900 differentially expressed genes described by Gattinoni et al9 in CD19-CAR–modified standard and TSCM-enriched products and naturally occurring CD8+ T-cell subsets from Gattinoni et al.9 (C). Gene set enrichment analysis (GSEA) on transcriptomes of CD19-CAR–modified standard and TSCM-enriched products using genes upregulated or downregulated in TSCM relative to TEM retrieved from Gattinoni et al9 as gene sets. (D) Schematic representation of the glycolytic pathway. In blue font, glycolytic enzymes and molecules whose genes were downregulated in CD19-CAR–modified TSCM-enriched cells compared with standard products (P < .05). In gray font, glycolytic enzymes not differentially expressed. DHAP, dihydroxyacetone phosphate; S/N, signal/noise.

CD19-CAR–modified TSCM have a transcriptome profile similar to their naturally occurring counterpart. (A) Hierarchical clustering of CD19-CAR–modified standard and TSCM-enriched products and naturally occurring CD8+ T-cell subsets from GSE23321 performed using a 900 gene list from Gattinoni et al. Red and blue colors indicate increased and decreased expression of 900 differentially regulated genes described by Gattinoni et al. Each column represents a sample and each row, a gene. (B) Principal component analysis (PCA) of the 900 differentially expressed genes described by Gattinoni et al in CD19-CAR–modified standard and TSCM-enriched products and naturally occurring CD8+ T-cell subsets from Gattinoni et al. (C). Gene set enrichment analysis (GSEA) on transcriptomes of CD19-CAR–modified standard and TSCM-enriched products using genes upregulated or downregulated in TSCM relative to TEM retrieved from Gattinoni et al as gene sets. (D) Schematic representation of the glycolytic pathway. In blue font, glycolytic enzymes and molecules whose genes were downregulated in CD19-CAR–modified TSCM-enriched cells compared with standard products (P < .05). In gray font, glycolytic enzymes not differentially expressed. DHAP, dihydroxyacetone phosphate; S/N, signal/noise.

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