VEGF-C is required for EMP colonization of the liver and primitive erythroblast distribution and maturation in circulation. (A) Quantification of CD41⁺c-kit⁺CD16/32⁺ EMPs in the E10.5 YS (n = 5-7 per group; mean ± SEM; P > .05). (B) Myelo-erythroid colony-forming ability of isolated CD41⁺c-kit⁺CD16/32⁺ EMPs, cultured in M3436 methocult medium in triplicates (n = 3-4 per group; mean ± SEM; P > .05). (C) Total E10.5 embryonic (including FLs) cells were cultured in M3436 methocult medium in duplicates. The colonies were counted at days 3, 10, and 14. The number of BFU-Es and CFU erythroid numbers are shown (n = 5-7 per group; mean ± SEM; *P < .05). (D) Representative flow cytometric analysis of CD41⁺c-kit⁺CD16/32⁺ EMPs in the E10.5 FL. Percentage of CD41⁺c-kit⁺ and CD41⁺c-kit⁺CD16/32⁺ cells. Quantification of the numbers of CD41⁺c-kit⁺CD16/32⁺ EMPs in the E10.5 FL (n = 5-10 per group; mean ± SEM; *P < .05). (E) Quantification of the numbers of CD41⁺c-kit⁺CD16/32⁺ EMPs in the E10.5 PB (n = 5-10 per group; mean ± SEM; P < .05). (F) Quantifications of the numbers of CD71⁺Ter-119⁻ proerythroblasts and CD71⁺Ter-119⁺ erythroblasts in YS at E10.5 (n = 6-8 per group; mean ± SEM; *P < .05). (G) Quantification of the number of CD71⁺Ter-119⁺ erythroblasts in PB and FL (n = 3-8 per group; mean ± SEM; *P < .05). (H) Representative microscopic images of Wright-Giemsa–stained cytospins from E12.5 WT and Vc^iΔR26 PB. Note multi- or binucleated erythroblasts (arrowheads) could be observed in E12.5 Vc^iΔR26 PB. Bar represents 25 μm.