American Society of Hematology

Figure 5.

$Figure 5. Actin network and NMII isoform localization. (A) Representative confocal microscopy images of in vitro–cultured day 9 control ERYs and ERYs treated with the indicated inhibitors. Cells were costained for NMIIA and NMIIB. DAPI was used to indicate the mitoticcells. Bar represents 10 μm. (B,C) Representative western blot of day 10 ERYs treated with/without cytochalasin D and latrunculin B. Cells were subjected to Triton X-100 fractionation and the soluble S and insoluble P fractions were probed for NMIIA, NMIIB, and β-actin. HSC70 was used as a loading control. The densitometric analysis of the blots is provided in supplemental Figure 16.$

Actin network and NMII isoform localization. (A) Representative confocal microscopy images of in vitro–cultured day 9 control ERYs and ERYs treated with the indicated inhibitors. Cells were costained for NMIIA and NMIIB. DAPI was used to indicate the mitoticcells. Bar represents 10 μm. (B,C) Representative western blot of day 10 ERYs treated with/without cytochalasin D and latrunculin B. Cells were subjected to Triton X-100 fractionation and the soluble S and insoluble P fractions were probed for NMIIA, NMIIB, and β-actin. HSC70 was used as a loading control. The densitometric analysis of the blots is provided in supplemental Figure 16.

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