LN-derived CLL cells have a phenotype associated with T-cell activation and migration. Matched LN and PB samples from 11 CLL patients were analyzed using multicolor flow cytometry, and the percent change between the LN and PB MFI calculated. (A) Compared with PB-derived CLL cells, LN-derived CD19⁺CD5⁺ CLL cells showed higher expression of the markers associated with antigen-presentation, co-stimulation, and activation: HLA-DR (Ai), CD5 (Aii), CD80 (Aiii), CD86 (Aiv), and CD69 (Av). (B) In addition, they have a phenotype associated with recent migration: raised CD49d (Bi) and CD38 (Bii), and reduced CXCR4 (Biii).