Figure 5.
Figure 5. PI3K inhibitor rescues rag1 expression. Morpholinos against fam49ab and ndrg1b can also affect thymus size. (A-D) rag1 expression in 4.5-dpf embryos treated with DMSO or 10 µM LY294002, and either uninjected or injected with 200 pg of fam49ab mRNA. (E) Plotted thymus area measurements from 3 independent experiments combined. n = 37 to 61 embryos per condition. (F-M) rag1 expression in 4.5-dpf embryos treated with indicated MOs and MOs together with mRNAs. (J) Quantification of (G-I), 3 independent experiments combined. n = 30 to 57 embryos per condition. (M) Quantification of (G-I), 3 independent experiments combined. n = 30 to 44 embryos per condition. *P < .05; **P < .01; ***P < .001; ****P < .0001, 1-way ANOVA. CoMO, control morpholino oligonucleotide.

PI3K inhibitor rescues rag1 expression. Morpholinos against fam49ab and ndrg1b can also affect thymus size. (A-D) rag1 expression in 4.5-dpf embryos treated with DMSO or 10 µM LY294002, and either uninjected or injected with 200 pg of fam49ab mRNA. (E) Plotted thymus area measurements from 3 independent experiments combined. n = 37 to 61 embryos per condition. (F-M) rag1 expression in 4.5-dpf embryos treated with indicated MOs and MOs together with mRNAs. (J) Quantification of (G-I), 3 independent experiments combined. n = 30 to 57 embryos per condition. (M) Quantification of (G-I), 3 independent experiments combined. n = 30 to 44 embryos per condition. *P < .05; **P < .01; ***P < .001; ****P < .0001, 1-way ANOVA. CoMO, control morpholino oligonucleotide.

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