fam49ab is expressed in hematopoietic organs in zebrafish.fam49ab expression in the aorta can be seen in (A-B) whole-mount embryo and (C-D) transverse section at 33 hpf (red arrowheads). (D) A magnification of the area is indicated, with a dotted rectangle in (C). Ao, aorta; NC, notochord; NT, neural tube. (E) Dorsal and (F) lateral views showing fam49ab expression at 4.5 dpf, and (G) dorsal and (H) lateral views showing fam49ab expression in the thymus (black staining) overlapping with rag1 expression (red staining, red and white arrowheads). (I) qRT-PCR showing fam49ab expression in ikaros⁺ or rag2⁺ thymocytes FACS sorted from 5 dpf embryos, and rag2⁺ cells sorted from dissected adult thymi. Graph shows 3 independent experiments combined.