Figure 5
![Figure 5. Increased PEAR1 expression during megakaryopoiesis parallels DNA methylation enrichment at CGI1. (A) Schematic representation of PEAR1 regions investigated in the methylation study indicated as black vertical boxes (“PCGI1” in CpG-island 1 [CGI1], “Pintron1” in intron 1 of PEAR1, “rs12041331”, “PCGI2” in CpG-island 2 [CGI2]). (B) Sequenom methylation estimates (% average) for PCGI1, Pintron1, and PCGI2 (bar representation, average ± standard deviation, n = 8). (C) Single CpG (dot line representation) during differentiation of CD34+ cells into MK precursors harvested at day 0 (D0), day 7 (D7), and day 14 (D14), and (D) of GG (n = 5) vs AG+AA (n = 3) methylation at day 14. *P < .05 one-way ANOVA.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/128/7/10.1182_blood-2015-11-682153/6/1003f5.jpeg?Expires=1722702461&Signature=d0yqBraZbBQOf-waSp-clqN10PH0~uZHiSV9JVbk8a0yrvRCwwM7x0nDtZyag023T3vya-StAV-hyztSX1GQ-iEsWnksC0lWyqRfT9ZtQt5ELItEB~LeUn0I2sj~D6zYDDIxCTBtQruMnF2d9PJ9Huf0biLA1RLgeIUpkGtE24U0VLgkDO7hUJfht6nBsY-vSyoNuG8H9gOvW9sFiqR4ahjIHDFAS~7Hy4SAoxyZ-2gXzCZEpsrKwwCqd-3OBDR3lnWdY2U9GErrECsCoPv9Y~Ev27c~rCbixUtW4SgQMupXqmasDF-rX2gW~OnITFWcKIUQsXZa2snyC668ueZGmw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Increased PEAR1 expression during megakaryopoiesis parallels DNA methylation enrichment at CGI1. (A) Schematic representation of PEAR1 regions investigated in the methylation study indicated as black vertical boxes (“PCGI1” in CpG-island 1 [CGI1], “Pintron1” in intron 1 of PEAR1, “rs12041331”, “PCGI2” in CpG-island 2 [CGI2]). (B) Sequenom methylation estimates (% average) for PCGI1, Pintron1, and PCGI2 (bar representation, average ± standard deviation, n = 8). (C) Single CpG (dot line representation) during differentiation of CD34+ cells into MK precursors harvested at day 0 (D0), day 7 (D7), and day 14 (D14), and (D) of GG (n = 5) vs AG+AA (n = 3) methylation at day 14. *P < .05 one-way ANOVA.
