Multimers from recombinant WT and mutant VWF splice variants secreted from HEK293T cells. 100% indicates homozygous transfections; 50% indicates transfections in the mock heterozygous state where HEK293T cells were transfected with 50% WT vector and 50% mutant vector. Lane 1, Abnormal 2A control. Lane 2, NPP control. Lane 3, 100% 23del. Lane 4, 50% 23del. Lane 5, 100% 26del. Lane 6, 50% 26del. Lane 7, 100% 23/26del. Lane 8, 50% 23/26del. Lane 9, 100% 33del. Lane 10, 50% 33 del. Lane 11, 100% 33-34del. Lane 12, 50% 33-34del.