Western blot analysis of plasma and recombinant VWF. (A) Family 1 plasma shows thick bands unable to separate potential low concentrations of mutants from similarly sized WT VWF. Lane 1, NPP. Lane 2, Patient V69 plasma. Lane 3, Patient V70 plasma. Lane 4, Recombinant WT VWF. Lane 5, Recombinant 23del VWF. Lane 6, Recombinant 26del VWF. Lane 7, Recombinant 23/26del VWF. Lane 8, Recombinant 23del lysate. (B) Family 2 plasma exhibits an additional band which size corresponds to 33del; 33-34del did not significantly separate from WT VWF. Lane 1, Recombinant WT VWF. Lane 2, Patient T151 plasma. Lane 3, Patient T152 plasma. Lane 4, NPP. Lane 5, Recombinant WT VWF. Lane 6, Recombinant 33del VWF. Lane 7, Recombinant 33-34del VWF. (C) Family 3 plasma shows no additional bands other than WT VWF. Lane 1, NPP. Lane 2, Patient V447 plasma. Lane 3, Patient V449 plasma. Lane 4, Recombinant WT VWF. Lane 5, Recombinant 38del VWF.