Differential patterns of ROS handling in genetically modified CD34+ iPS cells. (A) Genetically modified CD34+ NCRM1 iPSCs (1 × 104 cells per well) were loaded with 0.1 (v/v) resazurin for time-dependent spectrofluorimetric detection (530-nm excitation, 590-nm emission) of reduced resorufin as a quantitation of cellular redox coupling; data expressed as mean ± SEM of relative fluorescent units (N = 6); cellular BLVRB expression is shown, 20 μg lysates/lane; ****P < .0001. (B) Genetically modified NCRM1 iPSCs (1 × 105/mL) were treated (or not) with 200 μM TBHP for 1 hour at 37°C, followed by flow cytometric quantification of ROS-expressing cells after a 60-minute loading with cell-permeable 500 nM CellROX green as indicator (N = 6); *P < .05; **P < .01; ***P < .001; ****P < .0001.
