Design of transgenic mice with altered deoxyHb-binding sites on erythrocyte band 3. (A) The homologous recombination strategy to targeting the murine band 3 (Slc4A1) allele. The blue boxes represent the exons of human SLC4A1 knocked into the Slc4A1 locus. The deletion of the sequence encoding amino acids 12-23 is indicated by a smaller box. The red triangles represent loxP sites which flank a PGK-NEO gene to facilitate subsequent removal. (B) Schematic representation of erythrocyte band 3 proteins from normal human, wild-type mouse, and the 2 transgenic mice including 1 with humanized band 3 (w/ human Hb site) and the other lacking the deoxyHb-binding site on band 3 (w/o Hb site). w/, with; w/o, without.