Nonhematopoietic genes are ectopically activated in Setdb1-deficient HSPCs. (A) Sequential plots of genes according to fold expression changes in Setdb1^Δ/Δ LSK cells and GMPs from WT cells 2 weeks after the first injection of tamoxifen detected by RNA-seq. Representative genes with significant expression changes are indicated. (B) Enrichment of organ-specific genes in upregulated genes (≥×2.0) in Setdb1^Δ/Δ GMPs. The enrichment of genes preferentially expressed in each organ and tissue in the above gene set was calculated by ExAtlas software (http://lgsun.grc.nia.nih.gov/exatlas/). (C) A scatter plot showing the correlation between H3K9me3 levels and the expression levels of RefSeq genes in Setdb1^Δ/Δ GMPs relative to WT GMPs. The 183 genes showing reductions in H3K9me3 levels greater than 2-fold (below dotted line) are indicated in red dots. (D) Enrichment of organ-specific genes in 183 genes losing H3K9me3 greater than 2-fold in Setdb1^Δ/Δ GMPs among genes that showed H3K9me3 enrichment greater than 2-fold over the input signal in (C). The enrichment of genes preferentially expressed in each organ and tissue in the above gene set was calculated by ExAtlas software, as in (B).