Figure 4.
![Figure 4. HSF1A increased STAT3 function in AD-HIES patient EBV cells. EBV cells were treated with HSF1A (3 μM) for 48 hours, then stimulated with IL-21 (50 ng/mL). SOCS3 expression was measured by quantitative RT-PCR 120 minutes after stimulation (A). Total and phosphotyrosylated STAT3 and GAPDH were measured by Luminex 30 minutes after stimulation and results shown (B-D). STAT3 protein t1/2 determined (E-F; n ≥4 for each experiment). Comparisons of means marked by symbols *, **, #, ##, ^, ^^, ×, ××, ∞∞, ∨, ∨∨, +, &, &&, §, §§ indicate P < .01; ∞ indicates P < .05 ([A-D] analysis of variance [ANOVA]; [F] Student t test).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/128/26/10.1182_blood-2016-02-702373/5/blood702373f4.jpeg?Expires=1724259376&Signature=fp62QTRhpBOf3k-N2mUe7vjyhKTwCUlGtvCS9zoingBpMX0mFcEgppQ1o5pZwX0cEYGY3VFd8h3mE9REGFV~B7Rg8pVHHGaHVvcOgHMpiQtiY~Trtrd5REKJUDtIiQSGfwWHPM3tdxGdJllHZO3vfGiD8Sqwwdd~qX9Wi3y0HFSqu1O2q4qUzHfSzWgmQl9nVN7cQp7XyKUA4G8S5ncV3AKc7aNq6QrkaufeIevAJvwNa-zZ7-vDoQr6pzKdLaOVM7RdWif-JyNAlV5hPZgVxJ~UwLeyyFWF1mdnnOPdbRjxyap6~~96iJ699R-a84cZx5b0eH4JLM5lDUtrtWA7~A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
HSF1A increased STAT3 function in AD-HIES patient EBV cells. EBV cells were treated with HSF1A (3 μM) for 48 hours, then stimulated with IL-21 (50 ng/mL). SOCS3 expression was measured by quantitative RT-PCR 120 minutes after stimulation (A). Total and phosphotyrosylated STAT3 and GAPDH were measured by Luminex 30 minutes after stimulation and results shown (B-D). STAT3 protein t1/2 determined (E-F; n ≥4 for each experiment). Comparisons of means marked by symbols *, **, #, ##, ^, ^^, ×, ××, ∞∞, ∨, ∨∨, +, &, &&, §, §§ indicate P < .01; ∞ indicates P < .05 ([A-D] analysis of variance [ANOVA]; [F] Student t test).
